CONSULTATION ON METHODS FOR TREATING HIGH NITROGEN, AMMONIA, AND TOTAL NITROGEN IN DOMESTIC WASTEWATER

A. Introduction

1. Current status of the treatment system:

- Wastewater Type: Residential buildings, high-rise buildings.

- System Capacity: 100 m³/day.

- Aerobic Tank Volume: 60 m³.

- Nitrogen Levels: 1.5 times higher than the discharge standards.

- The system is operational and requires additional microbial seeding after the Tet holiday.

- Total nitrogen does not meet the QCVN 14:2008 – National Technical Regulation on Domestic Wastewater.

2. Treatment technology of the system: AO technology – Anoxic combined with Aerobic

3. Customer requirements: Achieve total nitrogen discharge standards according to QCVN 14:2008 – National technical regulation on domestic wastewater.

 

B. Wastewater characteristic

- Wastewater from residential buildings and high-rise buildings typically includes effluents from commercial centers, mini supermarkets, and daily water use activities of individuals.

- Domestic wastewater contains a high concentration of biodegradable organic matter, inorganic components, microorganisms, and pathogenic bacteria. The flow rate of wastewater is determined based on the number of staff, customers, and stalls...

 

C. Nitrogen Treatment Process using Bioclean ACF Nitro Activator

The process involves using microorganisms to influence the transformation of nitrogen species within molecular nitrogen. Nitrogen treatment in wastewater involves two main processes:

 

D. Nitrification process

The nitrification process involves the oxidation of ammonia (NH3, NH4+) to nitrite (NO2-) and then to nitrate (NO3-) under suitable conditions. This process is carried out by two groups of bacteria Nitrosomonas and Nitrobacter:

* Nitrosomonas Bacteria: convert ammonia to nitrite. This stage is called nitritation, resulting in te formation of NO2-

NH4+ + 3/2 O2 → NO2- + H2O + 2H+ + energy

* Nitrobacter Bacteria: convert nitrite to nitrate. This stage is called nitratation, resulting in the formation of NO3-

NO2- + ½ O2 →  NO3- + energy

 

E. Denitrification process

The denitrification process involves the removal of oxygen from nitrite and nitrate under the influence of anaerobic bacteria (denitrifying bacteria). The oxygen released from nitrite and nitrate is used to oxidize organic matter. This occurs in an anoxic tanks with denitrifying bacteria and some Bacillus species (B. subtilis, B.amyloliquifaciens, Bacillus licheniforms…)

NO3– → NO2– → NO →  N2O → N2

What are the benefits of BioClean ACF Nitro Activator? How does the effectiveness of the product impact the nitrogen treatment process?

 

F. Introduction to BioClean ACF Nitro Activator

Highlights:

- Bioclean ACF-NITRO ACTIVATOR (new name), Aquaclean NA (old name) is a microbial product designed for HIGH TOTAL NITROGEN REMOVAL and AMMONIA REDUCTION with the latest improved powdered formula.

- Origin: USA - Manufacturer: Sun & Earth Microbiology

- Ingredients: Contains two specialized microbial strains for the nitrification process:

• Nitrosomonas sp (convert Ammonia to Nitrite)
• Nitrobacter sp (convert Nitrite to Nitrate).

​Dosage and Usage instructions:

* Dosage:

- Initial dose (first week): 15 - 20 g/m3 of the treatment tank.

- Maintenance dose : 10 - 15 g/m3 of the treatment tank.

Note: 1ppm = 1mg/l =1g/m³

The dosage may vary depending on the level of pollution in the system.

* Usage instructions:

Bioclean ACF – NA is an aerobic microorganism and can be used in two ways:

     Method 1: Biomass cultivation with Aeration - This method provides optimal efficiency.

Preparation:

- Calculate the amount of microorganisims according to the initial or maintenance dose.

- Molasses

- Clean container

- Fish tank aeration pump strong enough to dissolve the microbial product.

- Clean water free of chemicals, chlorine, bleach, or disinfectants.

- The ratio of microorganisims: Molasses: Clean water is 1:1:8 ( 1 liter of microorganisms + 1 liter of molasses + 8 liters of clean, chlorine-free water). The amount of molasses can be adjusted based on customer requirements.

Biomass cultivation: Combine the calculated amounts of microorganisms, molasses, and clean water according to the determined ratio, then aerate using a fish tank aeration pump for 12 -14 hours, ideally for one day, to allow for biomass cultivation. After 12 - 24 hours (or one day) of aeration, distribute the mixture evenly around the aerobic tank (aeration tank) to initiate mictobial activity. Monitor and supplement nutrients C:N:P for the microorganisims in a ratio of 100:5:1. If the system is to large, it is optimal to divide one dose into 2 - 3 applications.

     Method 2: Direct application

- Similarly to the first method, calculate the required amount of microorganisms, then mix with water and molasses according to the ratio and distribute evenly around the surface of the aerobic tank to initiate microbial activity.

- Monitor and supplement nutrients C:N:P for the microorganisms in a ratio of 100:5:1. If the system is to large, it is optimal to divide one dose into 2 - 3 applications.

 

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